RE: CD-117

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From:Hewlett Bryan <HEWLETT@HHSC.CA>
To:'Histonet' <>, "'Nocito, Joseph'" <>


We have been using CD117 on FFPE sections for the past few years, using the
polyclonal antibodies from either Santa Cruz(C-19 cat# sc-168) or
Dako(A4502). The Dako Ab is absorbed against human. If you use the Santa
Cruz Ab be aware that you will have to add 10% heat inactivated human AB
serum to the diluent, to absorb. For more technical details see; Am. J.
Surg. Pathol. 23(4): 377-389, 1999.


> ----------
> From: 	Nocito, Joseph[]
> Sent: 	March 13, 2000 8:14 AM
> To: 	'Histonet'
> Subject: 	CD-117
> Hello netters,
> on a recent CAP survey for IHC, CD-117 was one of the antibodies
> requested,
> looking through the catalogs, I've spotted several clones and species.
> Because of budget restraints, I can't perform an adequate screening of
> three
> or four different CD-117 antibodies.  Does anyone use CD-117 on
> formalin-fixed, paraffin embedded human tissue? Is it worth trying? I've
> seen monoclonal and polyclonal antibodies. Which one works better.  I
> thank
> you in advance for your help.  Have a happy and safe day!! 
> Joe Nocito, B.S., HT(ASCP)QIHC
> Histology Supervisor
> Christus Santa Rosa Hospitals
> San Antonio, Texas 

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