RE: Anthracene Blue

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From:"Kellar, Eric" <kellarec@MSX.UPMC.EDU>
To:'Mark Phillips ' <phima048@student.otago.ac.nz>, "'histonet@pathology.swmed.edu '" <histonet@pathology.swmed.edu>
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The weakly soluble, polycyclic fluorescent, aromatic hydrocarbon -
Anthracine Blue SWR (C.I. 58605) aka..Alizarin Blue 2RC is far too mutagenic
to handle in any capacity. There are a wide variety of safer enhancing
hematoxylins that will demonstrate nuclear detail (Gill's, Harris', Mayer's
& Ehrlich's).

Place your stock on the back shelf and never open it. Better yet, have it
removed by a professional.



Eric C. Kellar
University of Pittsburgh Medical Center


-----Original Message-----
From: Mark Phillips
To: histonet@pathology.swmed.edu
Sent: 2/27/00 6:38 PM
Subject: Anthracene Blue

Hi everyone,
I am trying to stain thick sections of the anus. I have a reference
which uses Anthracene Blue in place of haematoxylin (apparently
anthracene blue is very similar to haematoxylin, just less intense
staining. Has anyone used anthracene blue recently? Does anyone have any
suggestions or comments about what it is like to use etc.
Thanks,
Mark
-- 
_________________________________________
"Only two things are infinite, the universe and human 
stupidity, and I'm not sure about the former." 
Albert Einstein 


Dept. of Anatomy, and 
Dept. of Surgery
School of Medical Sciences
University of Otago
DUNEDIN
NEW ZEALAND.

Ph. (03) 474-1049 (Home)
       (03) 474-0999   ext. 8847 (Surgery Dept.)
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       (021) 292-5602 (Mobile)

e-mail : phima048@student.otago.ac.nz
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