Hi Histonetters.
I know that many of you looking for a new cryostat would also like to
consider having the CryoJane Tape-Transfer system installed for the
preparation of thin, flat, wrinkle-free paraffin-quality frozen sections. We
have installed many systems in Microm cryostats. However, to date we have
not installed any systems in the Microm 560. I therefore want to caution you
that the internal chamber of the 560 may not permit installation of the
CryoJane mechanism in a way to preserve the CryoJane process. That process
requires that the frozen section remains frozen until the section is fixed.
The problem in the 560 is that the CryoJane  mechanism might have to be
installed  too close to the sliding door where warm room temperature air
enters the cryostat chamber.  The frozen section could melt when being
transferred  from the cold on to which it cut tape, to the cold adhesive
coated slide! Melting of the section at any point before it is fixed will
degrade the quality.

We are not sure that the above is necessarily so, because we have not had
the opportunity to actually install a CryoJane system in a 560 as yet. Our
comments are only based on a visual assessment. We will have an opportunity
very soon to actually install a system and run a test. We will post the
findings

Bernice
schiller@instrumedics.com