Dear Histonetters,
For those of you who do IHC stains and get slides that have been previously stained e.g. H&E, how do you get the coverslip film off the slides?  I know there is a quick method by placing the slides in acetone, but does this affect the antigenicity of those slides? 
Right now I soak them in Xylene, but it takes days to get the film unglued.  Glass coverslips come off rather quickly in xylene, it's just the film coverslip that takes forever.
Thanks for whatever info you can share.
Ann Maruska
Fairview-University Med Ctr
Mpls.  MN  55454
amarusk1@fairview.org