IHC on paraffin embbeded tissue

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From:J.Bruyntjes@voeding.tno.nl
To:HISTONET@PATHOLOGY.SWMED.EDU
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Dear all

We would like to do immunohistochemistry on rat lung tissue and we are
specially interrested in CD4, CD8 and some macrophage-markers sich as ED1
and Ed2. But we would like to have good morphology as well. I have found
some articles about IHC (rat and mice) on tissue fixed in
paraformaldehyde-lysine-peroidate and emdedded in low temp paraffin. 
But most of these articles are rather old (1985-1990). Is anyone familliar
with these techniques? Is it difficult to repeat and to get similar results?


Joost Bruijntjes
TNO Nutrition and Food Research Institute
Zeist
Holland
E-mail: J.Bruyntjes@voeding.tno.nl





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