IHC on paraffin embbeded tissue
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From: | J.Bruyntjes@voeding.tno.nl |
To: | HISTONET@PATHOLOGY.SWMED.EDU |
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Content-Type: | text/plain |
Dear all
We would like to do immunohistochemistry on rat lung tissue and we are
specially interrested in CD4, CD8 and some macrophage-markers sich as ED1
and Ed2. But we would like to have good morphology as well. I have found
some articles about IHC (rat and mice) on tissue fixed in
paraformaldehyde-lysine-peroidate and emdedded in low temp paraffin.
But most of these articles are rather old (1985-1990). Is anyone familliar
with these techniques? Is it difficult to repeat and to get similar results?
Joost Bruijntjes
TNO Nutrition and Food Research Institute
Zeist
Holland
E-mail: J.Bruyntjes@voeding.tno.nl
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