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<blockquote type=cite cite>Date: Wed, 08 Mar 2000 10:20:50 -0600<br>
From: Rebecca S Smith <bssvpisu@iastate.edu><br>
Subject: ATPase<br>
To: HistoNet Server <histonet@pathology.swmed.edu><br>
<br>
Hey you muscle experts! I have performed the wonderful ATPase stain
on mouse tissues with VERY little differentiation. Almost
nill. I seem to remember that different species have different pH's
that muscle typing is optimal at. I did the basic 9.4, 4.6 &
4.3. Is there a better set of pH's to try for a better shot at
actually muscle typing these mice. Thanks in advance. I know
someone's got the answer to this one!</blockquote><br>
Rebecca,<br>
<x-tab> </x-tab><x-tab> </x-tab>ATPase,
the bane of my life and a severe pain in the bottom. Works well in humans
but other species, well, can be a bit troublesome. Try the method by Joan
Round et al., 1980 Histochem J.<u>12</u>. 707-710. I've found it a good
reliable method for most species. But, you'll have to ring the changes
with the pH. Try the routine method from 9-10 and the acid reverse
methods from 4-5. You might even have to go to .05 changes for good
typing. I'm doing the same thing myself at the moment but on rabbits. You
think mice are bad, try bunnies.<br>
Ian.<br>
<br>
<br>
<br>
<br>
<div>Dr. Ian Montgomery,</div>
<div>West Medical Building,</div>
<div>University of Glasgow,</div>
<div>Glasgow,</div>
<div>G12 8QQ.</div>
<div>Tel: 0141 339 8855. Extn:6602.</div>
<div>Fax: 0141 330 2923</div>
e-mail: ian.montgomery@bio.gla.ac.uk
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