Frozen Cell Pellets

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From:"Kowsz, Kim P" <>
To:"''" <>
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Hi.  I am looking for someone who has experience freezing cell pellets for
H&E and immunohistochemistry.  I will be receiving 10e7 or 10e8 mouse cells
in 50 ml of media.  I plan to spin the cells down to a pellet and pour off
the media.  Next I would gently mix the cells with OCT and then freeze in
isopentane/dry ice.  Hopefully the pellet will pop out of the tube once
frozen.  Since I have never done this before, I am looking for advice and/or
alternative procedures that have proven successful.  I want to avoid and
freeze/thaw artifact!  Thanks.

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