-----Original Message-----
From: Mark V. Corl [mailto:mark.corl@labvision.com]
Sent: Thursday, 9 March 2000 4:01
To: Chewy71874@aol.com; histonet
Subject: Re: Cyclin D1


Hi-
This is a rather old and convoluted story, and ultimately a tricky antibody.
We
have been marketing this antibody for many years.  It has been a sore spot,
because it is very difficult to support technically.  We get it to stain,
others
do, and others do not.

Cyclin D1 is among many cyclins that are expressed during proliferation.
Therefore, highly proliferating tissues, like breast ca or tonsil will
express
this protein.  We have good success using breast ca in our QC lab.  However,
not
all of the breasts work that well.  This is what I have learned in dealing
with
various labs.  I am not sure of the reason, be it fixation or expression or
interpretation.

Anyways, in mantle cell lymphomas, there is an over expression of the cyclin
D1
that is associated with a gene translocation.  So there is a subtle increase
in
the amount of staining as compared to the control (meaning 3+ compared to
2+...
something like that).  There are several good papers on this, if you like we
can
fax them to you.  As I said, this is a very difficult antibody to work
with...

If my memory serves me correctly, the techs in Tom Grogan's lab have been
successful using combining a HEIR pretreatment and an enzyme pretreatment.
This
has allowed them for more consistent results.  In Clive Taylor's lab, they
actually use a cocktail of 2 different monoclonal antibodies to get
consistency.
Again, the results vary from lab to lab.

We have a a comprehensive data sheet available at this location on the web:
http://www.labvision.com/Products/Antibodies/Cell_Cycle/Cyclins/CyclinD1.htm
l

The recommended clone is DCS-6.  On our sight, you can see an image of the
stain,
and you can print the data sheet if you have adobe acrobat software with
your web
browser (interface).  If you cannot get it from the web, please send me an
e-mail
and we will fax the informatino to you.

I direct you to the data sheet because there is a very complete description
of
the Cyclin story, and many references.

Please let me know if I may assist you further.

Mark Corl
Lab Vision - NeoMarkers
Technical Support
http://www.labvision.com

Chewy71874@aol.com wrote:

> Some of you have written that you use brain, tonsil, or lymphoma for
Cyclin
> D1 control.  Doesn't it have to be mantle cell lymphoma?  What would it
stain
> in the brain and tonsil?  The spec sheet doesn't address what to use for
> control.
>
> Ellen