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From:Gayle Callis <uvsgc@msu.oscs.montana.edu>
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What I have noticed about Dierite is - it tends to crumble into minute
pieces, making a fine dust that I dislike anywhere around my tissues or
tissue sections.  
Bet that is a not good for the processor, plus carrys over into all the
rest of the solvents, creating another set of problems, fine grit.  

I wonder if it is really that important to have absolute 100% alcohol in
the last station.  I think there has been some recent discussion about
this, and it never seemed to affect my tissues or the way they sectioned
when I used run of the mill 100% ethanol, which is not pure 100% anyway,
more like 98%??? or thereabouts.  


Gayle Callis
Veterinary Molecular Biology
Montana State University
Bozeman MT 59717-3610
406 994-4705
406 994-4303

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