Cryosectioning
<< Previous Message | Next Message >>
| From: | "tony.whyte" <tony.whyte@bbsrc.ac.uk> |
| To: | histonet@pathology.swmed.edu (Reply requested) |
| Reply-To: | |
| Content-Type: | text/plain; charset = ISO-8859-1; Type = Text; name = "Cryosectioning.TXT"; x-dmw-oid = 2A864886F70F0301; x-dmw-btname = "PlainText" |
Hi all,
i'm having trouble sectioning whole frozen mouse embryos. They were snap
frozen in isopentane in liq N2 and mounted in OCT on cork. The trouble is
either that the section is compressing or else fragmenting. Using a new knife
hasn't helped the problem. Any ideas?
Tony W.
Cambridge
<< Previous Message | Next Message >>