Bone Marrow Decalcification

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From:Mary Lux <mlux@conpoint.com>
To:histonet@pathology.swmed.edu
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Hello Netters,
I would like to know the protocol for decaling bone marrow core biopsy
specimens.  What type of decalcifying agent used; how long of an exposure to
the reagent; what is the processor protocol.
We are using a week acid solution in the decal phase and are noticing
artifacts like shrunken nuclear material, pigmented material and
streaking/tearing when cutting the block.
Thanks in advance,
Tony Mesa
Histology Supervisor
Pathology Medical Services
:-) Reply to
SPOST@ALLTEL.NET





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