Dear all,

A basic question:

One of the antibodies I'm using appears to have a problem with Ig 
precipitate in the solution.  This appears on the slides as clumps or 
other unexpected deposits of chromagen (in this case, Vector 
VIP).  An obvious solution would be to centrifuge the antibody, so 
can someone suggest duration and rpm?  I'm a bit paranoid about 
spinning down my antibody along with the precipitate!

Thanks,

Liz Sabin.