water bath method for antigen retrieval. -Reply

<< Previous Message | Next Message >>
From:"Richard Cartun" <Rcartun@harthosp.org>
To:<tomers@home.com>, <HistoNet@Pathology.swmed.edu>
Date:Mon, 21 Jun 1999 15:06:21 -0400
Content-Type:text/plain; charset=US-ASCII

I have been experimenting with the "ISOTEMP 205" waterbath from Fisher Scientific.  I have used it mainly for the HercepTest, but have also used it for other markers including CD15, CD30, NSE, chromogranin, ER, PR, Ki-67, and others.  So far it has worked well.  I generally incubated slides at 95 degrees for 20 minutes (HercepTest - 40 minutes).  Immunoreactivity has been comparable to our steamer, but with less tissue loss.

R. Cartun

<< Previous Message | Next Message >>