water bath method for antigen retrieval. -Reply
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| From: | "Richard Cartun" <Rcartun@harthosp.org> |
| To: | <tomers@home.com>, <HistoNet@Pathology.swmed.edu> |
| Reply-To: | |
| Date: | Mon, 21 Jun 1999 15:06:21 -0400 |
| Content-Type: | text/plain; charset=US-ASCII |
I have been experimenting with the "ISOTEMP 205" waterbath from Fisher Scientific. I have used it mainly for the HercepTest, but have also used it for other markers including CD15, CD30, NSE, chromogranin, ER, PR, Ki-67, and others. So far it has worked well. I generally incubated slides at 95 degrees for 20 minutes (HercepTest - 40 minutes). Immunoreactivity has been comparable to our steamer, but with less tissue loss.
R. Cartun
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