From: | "Instrumedics, Inc." <info@instrumedics.com> |
To: | "HistoNet Server" <HistoNet@Pathology.swmed.edu> |
Reply-To: | |
Date: | Fri, 25 Jun 1999 10:27:55 -0400 |
Content-Type: | text/plain; charset="iso-8859-1" |
Hi Timothy, We have had very good results freezing core biopsies in the Gentle-Jane Snap Freezer. We dispense a small amount of CryoGel(or OCT) on the specimen holder and freeze to make a "safety" layer. This will assure that the specimen will not freeze into the surface of the specimen holder. Then we dispense another small amount of Gel on the frozen "safety" layer and orient the core(s) on the top of the gel. The heat extractor that has been stored in LN2 and it equilibrated to -196C is placed on the Gentle-Jane device. It falls at a controlled rate and contacts the cores first(best freezing) and in 8-10 sec. the cores and the embedding medium are snap frozen . The block is flat with the cores uppermost in the block which minimizes trimming to reveal a full face. This snap freezing method depends not only on the low temperature of LN2. but also on good thermal exchange that results from the highly polished chrome-plated 3/4 lb heat extractor. The morphology of the cores has been excellent with this method. Bernice schiller@instrumedics.com