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From:"David John Schmitz" <>
Date:Thu, 24 Jun 1999 10:04:48 -0400 (EDT)

Hi  everyone,

I am having a few problems with my histology.  I am trying to obtain a stage
series of axolotl embryos and I am having trouble because the have a tremendous
amount of fatty Yolk filled cells.  I have tried using bouinUs fix and smithUs
fix, and smithUs fix seems to work the best on the yolk. But, now I have a
number of embryos that have been fixed in buffered formalin, Can I postfix them
with SmithUs and have I work out OK?  If you have any other ideas of dealing
with the yolk problem, let me know .
I am also having a problem orientating the very early stage embryos and I heard
that agarose can be use for this.  Dose anyone know how to use agarose?

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