From: | "Luty, Carolyn" <cluty@is.mgh.mcgill.ca> |
To: | histonet <HISTONET@Pathology.swmed.edu> |
Reply-To: | |
Date: | Fri, 18 Jun 1999 09:40:00 -0400 |
Content-Type: | text/plain; charset="us-ascii" |
Paraformaldehyde---------------20gm Picric acid, saturated aqueous (double filtered)----------------150ml Heat to 60c to dissociate the paraformaldehyde. Add 2.52% aqueous NAOH dropwise to alkalinize the solution. Filter the solution and allow it to cool. Dilute the solution to 1000ml with phosphate buffer prepared as follows: NAH2PO4.H2o---------3.31gm NAH2PO4( anhydrous)-----18.88gm Distilled water------------1000ml The solution should have a final ph of 7.3 .Adjust as necessary. Hope this helps. Carolyn Montreal General Hospital.