| From: | "Luty, Carolyn" <cluty@is.mgh.mcgill.ca> |
| To: | histonet <HISTONET@Pathology.swmed.edu> |
| Reply-To: | |
| Date: | Fri, 18 Jun 1999 09:40:00 -0400 |
| Content-Type: | text/plain; charset="us-ascii" |
Paraformaldehyde---------------20gm
Picric acid, saturated aqueous
(double filtered)----------------150ml
Heat to 60c to dissociate the paraformaldehyde. Add 2.52% aqueous NAOH
dropwise to alkalinize the solution. Filter the solution and allow it to
cool. Dilute the solution to 1000ml with phosphate buffer prepared as
follows:
NAH2PO4.H2o---------3.31gm
NAH2PO4( anhydrous)-----18.88gm
Distilled water------------1000ml
The solution should have a final ph of 7.3 .Adjust as necessary.
Hope this helps.
Carolyn
Montreal General Hospital.