Uneven Keratin Staining

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From:Louri Caldwell <louri_c@hotmail.com> (by way of histonet)
Date:Fri, 2 Jul 1999 01:15:44 -0400
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I have found consistently good results using Dako's Wide-Spectrum Keratin
(catalog N1512)for an incubation period of 15 minutes following initial
antigen retrieval in Proteinase K (Dako catalog S3020) for 3 minutes.

When I initially assessed this antibody, I did find uneven staining using
both microwave treatment in Citrate Buffer and trypsin digestion.  Perhaps a
different retrieval method may solve the problem.

Hope this helps.

Louri Caldwell

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