I have found consistently good results using Dako's Wide-Spectrum Keratin
(catalog N1512)for an incubation period of 15 minutes following initial
antigen retrieval in Proteinase K (Dako catalog S3020) for 3 minutes.

When I initially assessed this antibody, I did find uneven staining using
both microwave treatment in Citrate Buffer and trypsin digestion.  Perhaps a
different retrieval method may solve the problem.

Hope this helps.

Louri Caldwell


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