Uneven Keratin Staining

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From:Richard Maliniak <maliniakrm@worldnet.att.net>
To:HistoNet <histonet@Pathology.swmed.edu>
Date:Fri, 25 Jun 1999 15:14:27 -0700
Content-Type:text/plain; charset=us-ascii

We are currently experiencing uneven...areas of Positive and areas of
Negative immunostaining on skin....with our DAKO keratin AE1/AE3. 
Protocol: primary antibody at 1:100 for 30 min at room temp, pepsin
pretreat(4mg pepsin/ml buffer, incubate 37C for 30 minutes),LSAB+
detector system(30 minutes at room temp)on DAKO autoimmunostainer. 
Our skins are formalin fixed for an assorted amount of times. Any

Thanks in Advance.

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