Uneven Keratin Staining
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From: | Richard Maliniak <maliniakrm@worldnet.att.net> |
To: | HistoNet <histonet@Pathology.swmed.edu> |
Reply-To: | |
Date: | Fri, 25 Jun 1999 15:14:27 -0700 |
Content-Type: | text/plain; charset=us-ascii |
We are currently experiencing uneven...areas of Positive and areas of
Negative immunostaining on skin....with our DAKO keratin AE1/AE3.
Protocol: primary antibody at 1:100 for 30 min at room temp, pepsin
pretreat(4mg pepsin/ml buffer, incubate 37C for 30 minutes),LSAB+
detector system(30 minutes at room temp)on DAKO autoimmunostainer.
Our skins are formalin fixed for an assorted amount of times. Any
suggestions?
Thanks in Advance.
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