Re: water bath method for antigen retrieval. -Reply

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From:"Tony Henwood" <>
To:<>, <>, "Richard Cartun" <>
Date:Fri, 25 Jun 1999 19:41:22 +0000
Content-Type:text/plain; charset=US-ASCII

Since water boils at less than 100oC, you may have problems with 
antigen retrieval (try recording the temperature when the water is 
boiling- it can be as low as 96oC!). If you replace the water in the 
water bath with oil or PEG (Can't remember the exact solution), you 
can obtain a temperature higher than 100oC. This should give you a 
chance of consistent antigen retrieval.

This was a trick we used when we used to do the protein binding 
radioassay for B12 and folate many years ago (in my biochem days)

Regards,   Tony

Tony Henwood
Senior Scientist
Anatomical Pathology
Royal Prince Alfred Hospital

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