Lynn,
            how are you cross-linking the collagen?
Barry

lynn_brennan@smtplink.Coh.ORG wrote:

> Hello everyone!
> We are currently growing 250-micron thick spinal cord slices on subbed, freshly
> made rat tail collagen coated, plastic coverslips, in a rollertube apparatus.
> The slices are taken from E12 rat embryos, (date of birth is E0), and are grown
> for several days invitro.  Using this paradigm, most of the slices do not stick
> well enough to culture for more than a couple of days.  Either endogenous
> collagenase is releasing the slices, or the substrate is wrong for this
> particular age.  E13 slices will adhere to this substrate for several weeks.
> Does anyone have suggestions that might help solve this problem?
> Thank you Lynn Brennan