Re: slices off coverslips

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From:Barry Rittman <>
Date:Thu, 17 Jun 1999 14:45:23 -0500
Content-Type:text/plain; charset=us-ascii

            how are you cross-linking the collagen?

lynn_brennan@smtplink.Coh.ORG wrote:

> Hello everyone!
> We are currently growing 250-micron thick spinal cord slices on subbed, freshly
> made rat tail collagen coated, plastic coverslips, in a rollertube apparatus.
> The slices are taken from E12 rat embryos, (date of birth is E0), and are grown
> for several days invitro.  Using this paradigm, most of the slices do not stick
> well enough to culture for more than a couple of days.  Either endogenous
> collagenase is releasing the slices, or the substrate is wrong for this
> particular age.  E13 slices will adhere to this substrate for several weeks.
> Does anyone have suggestions that might help solve this problem?
> Thank you Lynn Brennan

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