Re: rat and mouse immunostaining/compiling info

<< Previous Message | Next Message >> (Gayle Callis), (histonet)
Date:Thu, 24 Jun 1999 15:40:46 -0400 (Eastern Daylight Time)


I'd be glad to help as we have a full panel of CD markers but I do have
some questions/comments on your requirements.

Many of my incubation times/temp.s are VERY dependent on which
manufacturer's reagents I use.  Same is true for the antibodies.  It may
also be of benifit to show which modifications (of each technique) are
needed for cell culture and frozen sections.

It would also be valuable to note when or under which circumstances, in
the auther's experiance, antigen retrieval (method??) is used.

I realize why you don't want to use manuf. and trade names but some
times there is a real need to know.  After all these methods ARE all
working in the auther's lab.s so there will be NO NEGATIVE information
in the methods.  If for one reason or an other it doesn't work in
another lab. than they can trouble shoot with BOTH the auther and the
manuf. .

I do think that it is a great idea but it will take a lot of
time.........  ;-)

best regards,
Robert Schoonhoven
Laboratory of Molecular Carcinogenesis and Mutagenesis
Dept. of Environmental Sciences and Engineering
University of North Carolina
Chapel Hill, NC 27599
office 919-966-6343
   Lab 919-966-6140
   Fax 919-966-6123 

Don't go around saying the world owes you a living; the world owes you
nothing; it was here first. 
Mark Twain [Samuel Langhornne Clemens] (1835-1910) 

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