Hi John,

I get mine from Polysciences Inc. (I don't work for them-just use some of
their products.) and they are called Peel-A-Way molds. I use them to embed
frozen tissue, paraffin tissue, and JB-4 tissue and I have no complaints
about them. I think alot of other
suppliers are out there too and they will probably contact you directly. 

Karen

 

On Mon, 14 Jun 1999, John C. Dennis wrote:

> Gayle
> 
> Here's my first stupid question of the week:  where do I get these nifty
> sounding "disposable molds"?  Are they made in especial for frozen
> sections?
> 
> I'd look in the Fisher catalogue, but that catalogue scares hell out of
> me.
> 
> thanks.
> 
> John Carroll Dennis
> Anatomy, Physiology, and Pharmacology
> 109 Greene Hall
> Auburn University, AL  36849
> 
> 
> On Sun, 13 Jun 1999, Gayle Callis wrote:
> 
> > We embed a mouse brain (unfixed) in OCT, inside a Tissue tek disposable mold
> > and snap freeze it with a dry ice/isopentane slurry, and section at  -16C.
> > When you add isopentane to dry ice, it bubbles violently, when that stops
> > the correct temp has been reached for snap freezing of tissue.
> >  
> > You can also cool hexane with dry ice (put hexane in beaker, surround beaker
> > with  dry ice until hexane is cold (give it some time) and freeze with that,
> > a bit slower, but some prefer a tidge slower for brain, with excellent results
> > 
> > or try the dry ice slurry with hexane instead of isopentane.
> > 
> > Good luck
> > Gayle Callis
> > 
> 
> 
>