Re: axolotl (fwd)

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From:"Karen D. Larison" <>,
Date:Thu, 24 Jun 1999 12:01:06 -0800

We use a melted agar-sucrose solution to embed our zebrafish embryos for 
cryosectioning.  Protocols for this method can be found in The Zebrafish Book, 

There's also an article on embedding 7-day-old zebrafish in agarose for paraffin 
processing.  BioTechniques 25:614-618 (October 1998).

Hope this helps.

Karen in Oregon 

Date:          Thu, 24 Jun 1999 11:05:32 -0400 (EDT)
From:          "David John Schmitz" <>
Subject:       axolotl (fwd)

Forwarded message:
>From Thu Jun 24 10:55:18 1999
Date: Thu, 24 Jun 1999 10:04:48 -0400 (EDT)
Message-Id: <>
From: "David John Schmitz" <>
Subject: axolotl 
Content-Type: text/plain

Hi  everyone,

I am having a few problems with my histology.  I am trying to obtain a stage
series of axolotl embryos and I am having trouble because the have a tremendous
amount of fatty Yolk filled cells.  I have tried using bouinUs fix and smithUs
fix, and smithUs fix seems to work the best on the yolk. But, now I have a
number of embryos that have been fixed in buffered formalin, Can I postfix them
with SmithUs and have I work out OK?  If you have any other ideas of dealing
with the yolk problem, let me know .
I am also having a problem orientating the very early stage embryos and I heard
that agarose can be use for this.  Dose anyone know how to use agarose?


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