Dear Gayle and all interested Histonetters,

Vector NovaRED was developed as a red peroxidase substrate which is 
extremely sensitive and stable through (EtOH) dehydration and clearing 
procedures. We have not performed testing of this product with the 
multitude of aqueous based mounting media available, because we see that 
the other red peroxidase substrate, AEC, is used in this application. 
Since we did not evaluate the Vector NovaRED substrate with any aqueous 
mounting media, there is no mention of this on the specification sheet. 

As Gayle points out, the directions say to include a 5 min rinse in 
water. This is to remove any buffer traces before entering into the 
dehydration steps. This rinse can certainly be shortened and doesn't have 
to use running water. Vector NovaRED was tested in standing tap water for 
45 min at this stage of the procedure, and it did not disappear (i.e. is 
stable in tap water). 

I hope this information helps clarify the use of the product.

Sincerely,


__________

Craig Pow, Ph.D.

Vector Laboratories, Inc.
30 Ingold Road
Burlingame, CA 94010

Tel:  (650) 697-3600
Fax:  (650) 697-0339
E-mail:  craig@vectorlabs.com
Website:  http://www.vectorlabs.com