Re: Uneven Keratin Staining
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From: | Heike Grabsch <h.grabsch@uni-koeln.de> |
To: | Katri Tuomala <katri@istar.ca> |
Reply-To: | |
Date: | Sun, 27 Jun 1999 19:50:09 -0700 |
Content-Type: | text/plain; charset=us-ascii |
Regarding uneven staining with the cocktail AE1/AE3 in our hands: we
have seen several times no staining of regular liver tissue sections with
this antibody, which should be positive with this cocktail. We even
switched to another company, but had the same problems. May be, that
several components of the cocktail require different pre-treatment for
staining. We use the antibody usually with microwave pre-treatment.
Heike Grabsch
Dept. of Pathology
University of Duesseldorf
Germany
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