Re: Uneven Keratin Staining

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From:Heike Grabsch <>
To:Katri Tuomala <>
Date:Sun, 27 Jun 1999 19:50:09 -0700
Content-Type:text/plain; charset=us-ascii

Regarding uneven staining with the cocktail AE1/AE3 in our hands: we 
have seen several times no staining of regular liver tissue sections with 
this antibody, which should be positive with this cocktail. We even 
switched to another company, but had the same problems. May be, that 
several components of the cocktail require different pre-treatment for 
staining. We use the antibody usually with microwave pre-treatment.

Heike Grabsch
Dept. of Pathology
University of Duesseldorf

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