Re: Uneven Keratin Staining

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From:Katri Tuomala <>
To:Richard Maliniak <>
Date:Sun, 27 Jun 1999 22:59:44 -0400
Content-Type:text/plain; charset=us-ascii

Hi Richard,
Although AE1/AE3 antibody, being a monoclonal cocktail, is supposed to
be fairly wide spectrum and stain both squamous and simple epithelium,
my experience is that it misses some squamous eptihelium (maybe due to
formalin fixation). I never use it alone, particularly when doing a skin
lesion, but also run a wide spectrum polyclonal keratin antibody (Dako).
Over the years I've done immuno, I've come accross a few squamous cell
carcinomas totally negative for AE1/AE3, but nicely positive with the

Katri Tuomala
Anatomic Pathology
St.Joseph's Hospital
Hamilton, Ontario, Canada

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