Re: USURPED. Vector NovaRed fading; Tests & thoughts

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From:Katie B <bresee98@yahoo.com>
To:Histonet <histonet@Pathology.swmed.edu>
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Date:Tue, 29 Jun 1999 09:42:06 -0700 (PDT)
Content-Type:text/plain; charset=us-ascii


>   Dehydration in 3 X 100% alcohol is preferable to a graded series
>   because many (? most) anionic and cationic dyes are more easily
>   extracted by lower alcohols (70% etc, even 95%) than by 100%,
>   and you don't want to lose _any_ of the USURPED (Undisclosed
>   Secret Unidentified Red Peroxidase End Product). 

When I stain with May-Grunwald, I notice that if I don't move quickly
through the ethanols when I 'm dehydrating, I can loose quite a bit of
my stain.  Is this the reason why?  I think I may adjust to using 3 x
100% next time.

BTW - Applause for a great ranting against Souper Secret Ingredients!

===
Catherine "Katie" Bresee Bennett
Laboratory for Experimental Pathology
Department of Veterinary Pathology
Michigan State University

e-mail: bresee98@yahoo.com
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