Re: Smith's fixative
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From: | "J. A. Kiernan" <jkiernan@julian.uwo.ca> |
To: | |
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Date: | Thu, 24 Jun 1999 14:16:03 -0400 (EDT) |
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On Thu, 24 Jun 1999, Gayle Callis wrote:
> Could you give recipe for Smith's, not familiar with this one.
24-6-1999
The Microtomist's Formulary and Guide (P. Gray 1954)
has 5 different Smith's fixatives in it. Humason's
(5th edn) has one of these: a dichromate-formalin-acetic
mixture recommended for yolk-rich material. (In Gray
it's also for amphibian embryos.) This would be an
unstable mixture, even more so than Helly's. I'd expect
it to penetrate poorly and give good nuclei (including
mitoses & spindles), and poor cytoplasm.
Of course, there could be any number of fixatives with
a common name like Smith. They represent one kind of failure
in the system of eponymous naming. Another arises with ones
like Carnoy and Tellyesczky, where each individual described
at least 2 different fixatives.
John A. Kiernan,
Department of Anatomy & Cell Biology,
The University of Western Ontario,
LONDON, Canada N6A 5C1
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