Re: RE: Bone IHC- Elmer's Glue

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Date:Mon, 21 Jun 1999 09:37:44 -0400 (Eastern Daylight Time)

A note of caution with Elmer's glue.  Several years ago (OK, a lot of) when I
was in a clinical lab., we used Elmer's glue for those "Special Stains" that had
a tendency to cause the sections to float free from the slide.  I passed this on
to the membership of the Michigan Soc. of Hist. at a meeting.  If I recall (the
age vs. memory thing), Jerry Mead dicovered that if one did a Brown and Brenn or
Brown and Hopps (etc.) on these subed slides you WILL get false positives due to
bacteria growing in the glue (no, I don't remember if they are + or -).  He
contacted the manufacturer and they stated that yes, they were aware that there
may be bacteria in the glue but that they had no intention of doing anything
about it as it is, after all a wood glue, NOT intended for any  type of
biomedical use.  I will say that it worked great though :-)

-- Begin original message --

> From:
> Date: Sat, 19 Jun 1999 10:34:15 -0600
> Subject: RE: Bone IHC
> To:
> Cc:
>  Jim,
> as long as you are staining for lymphocytes this method should work for you.
> coat plus slides with a 5% mixture of elmer's white glue, mix with dionized
> water.  stand the slides up and allow to air dry.  use these slides to pick
> up your your sections, let the sections air dry on the glue coated slides
> then put the slides into a 60 d C oven for at least 60 min. overnight is
> prefered.  these sections stay on well, but if you are ever doing any IHC
> for collagen the glue will interfer with the staining, give false positive
> background.  t cell IHC should work without this background staining of the
> glue.
> patsy ruegg  

-- End original message --

best regards,
Robert Schoonhoven
Laboratory of Molecular Carcinogenesis and Mutagenesis
Dept. of Environmental Sciences and Engineering
University of North Carolina
Chapel Hill, NC 27599
office 919-966-6343
   Lab 919-966-6140
   Fax 919-966-6123 

**Suppose you were an idiot... And suppose you were a member of Congress ...
But I repeat myself.-Mark Twain**

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