Re: Photographic Film

<< Previous Message | Next Message >> (Philip Oshel)
Date:Tue, 22 Jun 1999 14:33:02 -0500
Content-Type:text/plain; charset="us-ascii"


Fuji or Kodak, use the slowest speed, lowest ASA you can. ASA 25 is good,
100 will produce good results.

Note!: if there is much vibration where your camera is, you may need to go
to a faster film to shorten your exposure times.

Use professional film, not consumer. The difference is that pro film is
refrigerated after it's made, so there is no color shift with aging. Keep
used film in your lab 'frig. for this reason.

You don't have to worry about daylight vs tungsten film since you're
shooting negatives and not transparencies, but if your photomicroscopy set
up controls color temperature, then try to shoot at 5500K (5500 deg), since
color film likes sunlight. Use neutral density filters to lower light
levels if needed.

Also: who's doing your printing? A film lab or someone used to histo shots?
If it's a film lab, then they won't know how to balance the color of your
sections, and you're likely to get weird results. If your camera back comes
off the scope, take the first one or two shots of a Caucasian person
outdoors, sun behind the camera. The automated developing and printing
machines are set to correctly balance Caucasian skin tones, and should keep
this setting for the rest of the roll. If your camera can not come off of
the 'scope, then when you send your film to be printed, include an image of
an H&E section with correct color balance. This will give the photo lab a
reference to use when balancing the color of your film when printing.


>My question is concerning photomicrography;
>    What brand of color 35mm film and ASA (film speed) is best suited for
>photographing H&E sections using an Olympus scope and camera setup?
>I would like to produce prints, not 2x2 projection slides.
>Thank You
>Patrick M. Haley
>Chief Technologist
>HistoTechNologies, inc.

****be famous! send in a tech tip or question***
Philip Oshel
Technical Editor, Microscopy Today
PO Box 620068
Middleton, WI  53562
Address for courier deliveries:
6319 Pheasant Lane  #A-12
Voice: (608) 833-2885
Fax: (608) 836-1969 (please make sure my name is on any fax)

<< Previous Message | Next Message >>