Re: Muscle

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From:rkline@emindustries.com
To:"Terry Hacker" <T.Hacker@har.mrc.ac.uk>
Reply-To:
Date:Wed, 23 Jun 1999 11:26:25 -0400
Content-Type:text/plain; charset=us-ascii

Terry,

Muscle can be brittle.  I always soaked them on a wet ice block until the
tissue started to swell and then was able to section the blocks very easily
without chatter.

Also, a trick someone taught me for cutting brittle tissue is to place the
block in the flotation bath for about 30 seconds and then back on wet ice
until cooled.  Take the first sections off the block face (if you don't
you'll hit brittle tissue again).  I had a lot a success cutting bloody
tissue with this method.

Rande Kline HT (ASCP)
EM Sciences




"Terry Hacker" <T.Hacker@har.mrc.ac.uk> on 06/23/99 08:06:58 AM

To:   "'Histonet'" <Histonet@Pathology.swmed.edu>
cc:
Subject:  Muscle




I have posed this question before and someone kindly gave me a
contact which I have since lost.
I am processing a number of fixed(formol saline) mouse muscles to
paraffin wax to look for potential myopathies. The trouble is that
there is a certain amount of artefact (fibre splitting,chattering
etc.) which is making it difficult to assess any true pathology and
because of the numbers I need a quick easy method as an initial look
before progressing to frozens/enzymes etc.
Any tips/ideas would be gratefully received.

Terry.

Terry Hacker,
Medical Research Council,
Harwell,
Oxfordshire,
U.K.








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