Re: Fixed frozen section adhesion

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From:"J. A. Kiernan" <>
To:"John C. Dennis" <>
Date:Wed, 30 Jun 1999 10:06:16 -0400 (EDT)
Content-Type:TEXT/PLAIN; charset=US-ASCII

On Tue, 29 Jun 1999, John C. Dennis wrote:

> My fixed frozen rat brain sections all fell off the slides when I tried to
> stain them.  I'd let them air dry for 2 mins.

  If these are thick frozen sections that you handle with a
  paintbrush or a glass hockey-stick (rather than cryostat
  sections collected directly onto glass), then they need
  to air-dry properly - at least an hour. It's better to
  do this slowly than to try to speed up the process on a
  warm hotplate. If they dry too quickly the edges curl
  up and cracks may form.

 John A. Kiernan,
 Department of Anatomy & Cell Biology,
 The University of Western Ontario,
 LONDON,  Canada  N6A 5C1

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