Re: Circulation time of BRDU in a rat

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From:"John C. Dennis" <>
To:Jamie Erickson <>
Date:Thu, 24 Jun 1999 16:06:54 -0500 (CDT)
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I THINK the original study was done in the '60's but I may be thinking of
another paper altogether.  Nonetheless, this reference is good enough
and/or will lead you to the original work.

Boswald et al., (1990).  Tracer dose and availability time of thymidine
and bromodeoxyuridine:  application of bromodeoxyuridine in cell kinetic
studies.  Cell Tissue Kinet.  23:169-181.

I use 50 ug/g BrdU IP for 1 hr.  According to the lit., 50 ug is above the
tracer dose but there's no evidence of damage at that dose.  It is all in
the liver (or in mitotic cells' DNA) by 1 hr.

You might also look in:

Skalko et al. (1971).  The teratogenic response of mouse embryos to
5-bromodeoxyuridine.  Teratology. 4:87-94.


John Carroll Dennis
Anatomy, Physiology, and Pharmacology
109 Greene Hall
Auburn University, AL  36849

On Wed, 23 Jun 1999, Jamie Erickson wrote:

> HI All,
>       I know this was discussed sometime ago but could someone tell me the circulation time for BRDU, I believe the dose of BRDU given was 500 ug/kg.  I'm doing a study to evaluate BRDU stained slides of Rat colon and need this info. Also how do people evaluate these slides, is it by number of positive cells/ crypt (how many do you count /slides?) . There seems to be two things to evaluate in these slides migration up the Crypt and proliferation, what's the best way to  evaluate both of these??
> If someone could help I'd really appreciate it.
> Jamie Erickson

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