Re: Circulation time of BRDU in a rat

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From:Katie B <bresee98@yahoo.com>
To:Jamie Erickson <JErickson@genetics.com>, HistoNet@Pathology.swmed.edu
Reply-To:
Date:Thu, 24 Jun 1999 05:47:25 -0700 (PDT)
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My understanding is that BrdU, after a single injection, circulates for
less than 2 hours.  But I don't have a reference for that.  (I use 50 -
60 mg/kg body weight, which is about the same as you.)

I evaluate my BrdU labeling index 2 ways:

1.  As a percent of all the cells in the region of interest.  (# BrdU
positive divided by total # cells)

2.  Our studies are of airway epithelium so we also measure the BrdU
labeling as a Unit Length Labeling Index (ULLI) which is the number of
positive cells divided by the length of the underlying basal lamina. 
You could probably do the same thing in the colon.

For every study, both indices are measured.  This is to compensate for
any cell death or hyperplasia which may have occured when compairing
between exposure groups.

--- Jamie Erickson <JErickson@genetics.com> wrote:
> HI All,
>       I know this was discussed sometime ago but could someone tell
> me the circulation time for BRDU, I believe the dose of BRDU given
> was 500 ug/kg.  I'm doing a study to evaluate BRDU stained slides of
> Rat colon and need this info. Also how do people evaluate these
> slides, is it by number of positive cells/ crypt (how many do you
> count /slides?) . There seems to be two things to evaluate in these
> slides migration up the Crypt and proliferation, what's the best way
> to  evaluate both of these??




===
Catherine "Katie" Bresee Bennett
Laboratory for Experimental Pathology
Department of Veterinary Pathology
Michigan State University

e-mail: bresee98@yahoo.com
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