Re: A quickmorphology stain for paraffin sections?

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From:Tim Morken <> (by way of histonet)
Date:Fri, 2 Jul 1999 01:15:07 -0400
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If the structure is fairly large you can use a pseudo-interference contrast
illumination method to see structure in the section. Just move the objective
of the microscope slightly to one side of its normal position and you can
see 3D structure without doing any deparaffinizing or staining. You will be
surprised how much detail you can make out. This is a great method for
finding gloms on kidney frozens.

Tim Morken, B.A., EMT(MSA), HTL(ASCP)
Infectious Disease Pathology
Centers for Disease Control
1600 Clifton Rd.
Atlanta, GA 30333


Phone: (404) 639-3964
FAX:  (404)639-3043

----Original Message Follows----
From: "Barry Rittman" <>
To: histonet <>
Subject: Re: A quickmorphology stain for paraffin sections?
Date: Wed, 30 Jun 1999 12:15:28 -0500

                 we used to use a cotton ball moistened with dilute
blue to wipe over the surface of the block. This gave us a good idea of the
tissue at that level and helped greatly in the orientation.
If you prefer  you can always place a cut section on a slide and add several
drops of dilute aqueous methylene blue (say 0.05-0.1%), this also works
No need to mount the section.
Barry wrote:

 > This is probably a silly question but ... I was wondering is there any
 > to quickly stain paraffin sections so that I can evaluate whether or not
 > need to cut further into the block.  We are looking for something very
 > specific and every section counts but to section the whole block would
 > hours of time not to mention many many expensive slides?  Can you quick
 > stain sections without deparaffinizing?
 > Andrea

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