Re: A quickmorphology stain for paraffin sections?

<< Previous Message | Next Message >>
From:"Patrick M. Haley " <> (by way of histonet)
Date:Fri, 2 Jul 1999 00:34:23 -0400
Content-Type:text/plain; charset="us-ascii"

I have used this technique when searching for glomerulae when sectioning
kidney bxs:

Mount the section on the slide as usual.
Place the slide on the microscope stage, use the 10x objective.
Close the condenser aperture down, and lower the entire condenser away from
the microscope stage.
What should result is a slightly out of focus image of the unstained tissue
You  may have to "tweek" the settings of the aperture and condenser in order
to realize an image.
 This works well for large structures such as the glomerulus in the nephron
a kidney.
I think placing polarizing filters on your scope may also allow you to see
basic morphology, but I am not sure.
I hope this helps. Good Luck!!

Patrick M. Haley B.S. HTL(ASCP)
Chief Technologist
HistoTechNologies, inc.

-----Original Message-----
From: <>
To: HistoNet Server <>
Date: Wednesday, June 30, 1999 9:50 AM
Subject: A quickmorphology stain for paraffin sections?

>This is probably a silly question but ... I was wondering is there any way
>to quickly stain paraffin sections so that I can evaluate whether or not I
>need to cut further into the block.  We are looking for something very
>specific and every section counts but to section the whole block would take
>hours of time not to mention many many expensive slides?  Can you quick
>stain sections without deparaffinizing?

<< Previous Message | Next Message >>