*Leishman's Stain:

Leishman's stain was originally developed by Scottish bacteriologist 
Gen. Sir W.B. Leishman at the turn of the century.

Swirl 500 ml absolute methanol in dry flask. Add 1.0 g Leishman powder stain
to swirling methanol. Continue to swirl for 2-4 min. at moderate rate. Let
sit for 15 min. Filter into dry brown bottle. Store from heat and light.
Shake well and filter before use. 

A buffer of pH 6.7-7.0 is also recommended for some G-band chromosal
staining. Giemsa powder can also be substituted for Leishman powder. Stain
improves with age.

*Leishman's Powder is available from Sigma #L-6254.



Eric C. Kellar
Histology/Immunohistochemistry
University of Pittsburgh Medical Center



-----Original Message-----
From: Katie B
To: Histonet Server
Sent: 6/17/99 5:19 PM
Subject: Leishmans Stain

I have a visiting scientist here (from Scotland?)who is looking at lung
lavage cytospins from horses.  She wants to do something called a
Leishmans stain in order to better identify mast cells and basiophils. 
She stains normally with the Leishmans for 2 min then dilutes it with
di water and stains for 8 min then rinses completely in water.  It's
not too different from our Diff Quick, but less pink.  This stain is
what she is acostomed to looking at, but she doesn't have a recipe. 
Anyone out there in Histoland know what this stuff is?

As always, thanks for all the knowledge out there!

-Katie



===
Catherine "Katie" Bresee Bennett
Laboratory for Experimental Pathology
Department of Veterinary Pathology
Michigan State University

e-mail: bresee98@yahoo.com
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