RE: Fixed frozen section adhesion

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Date:Fri, 2 Jul 1999 01:15:12 -0400
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It depends on what you are doing with the sections.  Silane coated slides
(plus slides) may be all you need to keep the brain (notoriously hard to
stick) sections on the slide, but if that doesn't work you could prepare
some glue coated slides ahead of time and use them to pick up the section,
they will stick, also the tape transfer system which uses polymer coated
slides will work well.
Patsy Ruegg

		-----Original Message-----
		From:	John C. Dennis []
		Sent:	Tuesday, June 29, 1999 2:56 PM
		To:	HistoNet Server
		Subject:	Fixed frozen section adhesion

		Dear Folks

		My fixed frozen rat brain sections all fell off the slides
when I tried to
		stain them.  I'd let them air dry for 2 mins.

		Can someone please tell me how to stick those babies on
there?  Longer
		time in air perhaps?

		Thanks for all the help, by the way, on the OCT question I
had last week.

		John Carroll Dennis
		Anatomy, Physiology, and Pharmacology
		109 Greene Hall
		Auburn University, AL  36849

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