RE: Fixed frozen section adhesion

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From:"April Esquibel" <>
To:"'John C. Dennis'" <>, "'HistoNet Server'" <>
Date:Tue, 29 Jun 1999 21:36:20 -0700
Content-Type:text/plain; charset="iso-8859-1"

Dear John,

What kind of slides are you using?  Silanized slides work great for frozen.

April Esquibel
DAKO Corporation
Technical Service Representative 
800-400-3256 ext. 5338

-----Original Message-----
From: John C. Dennis []
Sent: Tuesday, June 29, 1999 1:56 PM
To: HistoNet Server
Subject: Fixed frozen section adhesion

Dear Folks

My fixed frozen rat brain sections all fell off the slides when I tried to
stain them.  I'd let them air dry for 2 mins.

Can someone please tell me how to stick those babies on there?  Longer
time in air perhaps?

Thanks for all the help, by the way, on the OCT question I had last week.

John Carroll Dennis
Anatomy, Physiology, and Pharmacology
109 Greene Hall
Auburn University, AL  36849

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