RE: Bone IHC

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Date:Sat, 19 Jun 1999 10:34:15 -0600
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as long as you are staining for lymphocytes this method should work for you.
coat plus slides with a 5% mixture of elmer's white glue, mix with dionized
water.  stand the slides up and allow to air dry.  use these slides to pick
up your your sections, let the sections air dry on the glue coated slides
then put the slides into a 60 d C oven for at least 60 min. overnight is
prefered.  these sections stay on well, but if you are ever doing any IHC
for collagen the glue will interfer with the staining, give false positive
background.  t cell IHC should work without this background staining of the
patsy ruegg  

-----Original Message-----
From: Jim Manavis
To: Histonet
Sent: 6/17/99 7:12 PM
Subject: Bone IHC

I am after some advice on immunohistochemistry staining on bone
sections.  I
am currently trying to stain for T cells in rat ankle joints (paraffin
sections).  I need to use
antigen retrieval for the antibody and am having problems with the bone
coming off the slides.  We have tried a number of slide coatings, with
gelatin and Dako silanised slides being the best so far.  Our best
retrieval method so far has been 20 minutes in the autoclave.  The
amount of
tissue staying on the slides has definitely improved, but is not
Does any one have advice on IHC on bone sections.


Jim Manavis
Adelaide, South Australia

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