Leishmans Stain

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From:Katie B <bresee98@yahoo.com>
To:Histonet Server <histonet@Pathology.swmed.edu>
Date:Thu, 17 Jun 1999 14:19:39 -0700 (PDT)
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I have a visiting scientist here (from Scotland?)who is looking at lung
lavage cytospins from horses.  She wants to do something called a
Leishmans stain in order to better identify mast cells and basiophils. 
She stains normally with the Leishmans for 2 min then dilutes it with
di water and stains for 8 min then rinses completely in water.  It's
not too different from our Diff Quick, but less pink.  This stain is
what she is acostomed to looking at, but she doesn't have a recipe. 
Anyone out there in Histoland know what this stuff is?

As always, thanks for all the knowledge out there!


Catherine "Katie" Bresee Bennett
Laboratory for Experimental Pathology
Department of Veterinary Pathology
Michigan State University

e-mail: bresee98@yahoo.com
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