Immunohistochemistry in Bone Marrow Aspirates??

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Date:Fri, 25 Jun 1999 15:04:31 -0400
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     Hi Histonetters!  Any suggestions for a good protocol to fix and decalcify
bone marrow aspirates prior to processing and embedding in paraffin for
subsequent immunohistochemistry.  I have not worked with bone/bone marrow in the
past; although I did consult Sheehan's Histotechnology book and realize that
most conventional decalcifying methods may destroy endogenous enzyme activity.
Any thoughts or ideas on the subject???  Thank you very much as always!!

Jennifer Hoover
Transplantation Biology
Novartis Pharmaceuticals
Summit, NJ

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