Frozen sections--unfixed

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From:"John C. Dennis" <>
To:HistoNet Server <>
Date:Fri, 18 Jun 1999 14:45:31 -0500 (CDT)
Content-Type:TEXT/PLAIN; charset=US-ASCII

I forget which stupid question for the week this is but:

I'm trying to work up some immunocyto. stuff with fresh, unfixed tissue.

Would someone supply a ref. for protocols on this?

So far I've:

Cut sections
Let them air dry for approx. 2 minutes.
Put them into 70% EtOH o/n at 4 deg. C.
Brought them back to water and run an NCAM assay.  So far so good.

I'd appreciate anyone who could give me additional steps/improvements.

John Carroll Dennis
Anatomy, Physiology, and Pharmacology
109 Greene Hall
Auburn University, AL  36849

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