Freezing tissue in 70% ethanol
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From: | "Patrick M. Haley " <pmhales@cybergap.net> |
To: | "Histonet" <HistoNet@Pathology.swmed.edu> |
Reply-To: | |
Date: | Tue, 29 Jun 1999 17:07:25 -0700 |
Content-Type: | text/plain; charset="iso-8859-1" |
Has anyone out there in Histoland ever heard (or perhaps done this) of
placing tissue in 70% ethanol, and then freezing to -80 degrees C? I was
speaking to a researcher today, who has some tissue (bone with soft tissue
I believe) samples stored in this manner.
My first fear is artifact from the freezing/thawing process.
How does this storage effect staining, both special and IHC?
Thank you
Patrick M. Haley B.S. HTL(ASCP)
Chief Technologist
HistoTechNologies, inc.
www.histology.net
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