Freezing tissue in 70% ethanol

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From:"Patrick M. Haley " <>
To:"Histonet" <>
Date:Tue, 29 Jun 1999 17:07:25 -0700
Content-Type:text/plain; charset="iso-8859-1"

Has anyone out there in Histoland ever heard (or perhaps done this) of
placing tissue in 70% ethanol, and then freezing to -80 degrees C? I was
speaking to a researcher today, who has some tissue  (bone with soft tissue
I believe) samples stored in this manner.
My first fear is artifact from the freezing/thawing process.
How does this storage effect staining, both special and IHC?

Thank you

Patrick M. Haley B.S. HTL(ASCP)
Chief Technologist
HistoTechNologies, inc.

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