Desmin in mouse

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From:Greg Dobbin <dobbin@Upei.CA>
Date:Tue, 29 Jun 1999 15:49:04 -0400
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Hello All,

I'll be REALLY glad when the IHC in mice and rats info. is compiled!

I am trying to get mouse tissue to stain for desmin; with minimal 
success so far. There is just a hint of a positive reaction, and 
surprisingly it is slightly better without ag retreival (tissues are 
FFPE- 5um). However the rx is just too weak overall. Is there 
anything peculiar about IHC staining for desmin (in mice) that 
I should know about? (I used citrate buffer in steam for HIER
and a polyclonal, rabbit anti-desmin).

                                                             Cheers!  Greg
Greg Dobbin
Pathology Lab
Atlantic Veterinary College, U.P.E.I.
550 Unviversity Ave.
Charlottetown, P.E.I.
C1A 4P3
Phone: (902)566-0744
Fax: (902)566-0851

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