Desmin in mouse
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From: | Greg Dobbin <dobbin@Upei.CA> |
To: | histonet@Pathology.swmed.edu |
Reply-To: | |
Date: | Tue, 29 Jun 1999 15:49:04 -0400 |
Content-Type: | text/plain; charset=US-ASCII |
Hello All,
I'll be REALLY glad when the IHC in mice and rats info. is compiled!
I am trying to get mouse tissue to stain for desmin; with minimal
success so far. There is just a hint of a positive reaction, and
surprisingly it is slightly better without ag retreival (tissues are
FFPE- 5um). However the rx is just too weak overall. Is there
anything peculiar about IHC staining for desmin (in mice) that
I should know about? (I used citrate buffer in steam for HIER
and a polyclonal, rabbit anti-desmin).
Cheers! Greg
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Greg Dobbin
Pathology Lab
Atlantic Veterinary College, U.P.E.I.
550 Unviversity Ave.
Charlottetown, P.E.I.
C1A 4P3
Phone: (902)566-0744
Fax: (902)566-0851
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