undecalcified bone

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From:Gayle Callis <uvsgc@msu.oscs.montana.edu>
Date:Thu, 03 Jun 1999 08:49:54 -0600

Even if you put murine bone in polymethylmethacrylate, you will get 25%
shrinkage, which is comparable to paraffin (have a publication on this,
a comparison!)  Bone frozen sections are easiest done with an
Instrumedic Cryojane Tape Transfer system on snap frozen bone and you will 
need a tungsten carbide d profile knife.  Both are expensive, sectioning
system is approx $7000 and knives from DDK are approx $950, not to mention
a cryostat.  
However, the results are well worth the investment, and saves a good deal
of grief.

Gayle Callis

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