trypsin treatment for overfixed tissue

From:	Inka Tertinegg <inka@playfair.utoronto.ca>
To:	Histonet@Pathology.swmed.edu
Reply-To:
Date:	Fri, 04 Jun 1999 17:02:46 -0400
Content-Type:	text/plain; charset="us-ascii"

Hi,
	I' recently seen a couple of references to trypsin pretreatment of
overfixed tissue. Would anyone have a protocol for this that they could
share?(ie. concentration of trypsin, buffer and time of treatment).

	Thanks


	
Inka Tertinegg
University of Toronto
(416)603-5800 ext 2850
inka@playfair.utoronto.ca

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