trypsin treatment for overfixed tissue

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From:Inka Tertinegg <>
Date:Fri, 04 Jun 1999 17:02:46 -0400
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	I' recently seen a couple of references to trypsin pretreatment of
overfixed tissue. Would anyone have a protocol for this that they could
share?(ie. concentration of trypsin, buffer and time of treatment).


Inka Tertinegg
University of Toronto
(416)603-5800 ext 2850

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