smooth muscle alpha actin
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From: | Sylvia Loo <sloo@prl.pulmonary.ubc.ca> |
To: | histoNet@Pathology.swmed.edu |
Reply-To: | |
Date: | Thu, 03 Jun 1999 16:36:20 -0700 |
Content-Type: | text/plain; charset=US-ASCII |
Hello there,
Does anyone out there have experience with non-specific nuclear
staining artifact?
I've been having problem with immunostaining smooth muscle alpha
actin (Sigma A-2547) on cultured human smooth muscle cells.
I did a few optimizing test on the antibody. It was working fine at
1:400, stained the cytoplasm nicely and nice & blue counter stained
nuclei with haematoxylin. The antibody's been frozen in aliquotes.
Three four months later I use it again, it stains all nuclei dark brown. All
nuclei.
Any suggestion?
Thanks ahead of time
Sylvia Loo
University of British Columbia
Dept of Pathology & Lab Medicine
Vancouver, BC
Canada
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