smooth muscle alpha actin

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From:Sylvia Loo <>
Date:Thu, 03 Jun 1999 16:36:20 -0700
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Hello there,
Does anyone out there have experience with non-specific nuclear 
staining artifact?
I've been having problem with immunostaining smooth muscle alpha 
actin (Sigma A-2547) on cultured human smooth muscle cells. 
I did a few optimizing test on the antibody. It was working fine at 
1:400, stained the cytoplasm nicely and nice & blue counter stained 
nuclei with haematoxylin.  The antibody's been frozen in aliquotes. 
Three four months later I use it again, it stains all nuclei dark brown. All 
Any suggestion?
Thanks ahead of time

Sylvia Loo
University of British Columbia
Dept of Pathology & Lab Medicine
Vancouver, BC

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