pos control generation

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From:Brandon Schanbacher <schanbacher.2@osu.edu>
To:"'histonet@pathology.swmed.edu'" <histonet@Pathology.swmed.edu>
Date:Tue, 08 Jun 1999 10:22:51 -0400
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Hello all,
I have a question concerning generating a positive control.  I'm using
an iNOS antibody and am trying to get a slide with not just positive
staining, but maximal staining.  I have iNOS protein and would like some
way to get it to stick to a slide or onto a tissue.  I've tried just
drying it onto a slide, but expectedly, most of it gets washed of in the
buffer rinses.  Is there a way I could incubate a section in the protein
and expect it to stay there through the staining?  The reason behind
this is to get a maximal level of staining intensity for image analysis
purposes.  Any suggestions are appreciated.


Brandon Schanbacher
Division of Pharmacology
Ohio State University

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