pos control generation
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From: | Brandon Schanbacher <schanbacher.2@osu.edu> |
To: | "'histonet@pathology.swmed.edu'" <histonet@Pathology.swmed.edu> |
Reply-To: | |
Date: | Tue, 08 Jun 1999 10:22:51 -0400 |
Content-Type: | text/plain; charset=us-ascii |
Hello all,
I have a question concerning generating a positive control. I'm using
an iNOS antibody and am trying to get a slide with not just positive
staining, but maximal staining. I have iNOS protein and would like some
way to get it to stick to a slide or onto a tissue. I've tried just
drying it onto a slide, but expectedly, most of it gets washed of in the
buffer rinses. Is there a way I could incubate a section in the protein
and expect it to stay there through the staining? The reason behind
this is to get a maximal level of staining intensity for image analysis
purposes. Any suggestions are appreciated.
Thanks,
Brandon Schanbacher
--
Division of Pharmacology
Ohio State University
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