more on bone frozen sections

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Date:Thu, 03 Jun 1999 12:11:03 -0600
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I hope you do not get "shoot down" for expressing your opinion and
experience here.  I think that we all are just trying to do that.  

I have a lot of experience cutting frozen bone sections without
decalcification.  I even had some good results using Dodd's method of
embedding the bone in polyvinyl alcohol (which sets up much harder than OCT)
but this was on fairly small trephine samples of bone marrow biopsies.  A
whole rat femur is another thing all together.  Believe me, I am the first
to try something without buying expensive equipment or kits, etc.  I make up
my on GMA plastic because it is so much cheaper than using kits and I get
better results.  I use only D profile tungsten carbide knives for bone
sectioning, but I could not get sections of whole rat femur without using
the tape transfer method, and it did really burn me to have to buy the tape

This is my experience.  If you can get good frozen sections from calcified
rat femur without using something like a tape transfer system then you are a
better man than me and I would hope that you would do a workshop and show us
all how to do it.

Patsy Ruegg

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